Preparation of clarithromycin-loaded chitosan-coated PLGA nanoparticles with high antimicrobial activity with low dose use and evaluation of antimicrobial activity

Akyıl E., Karaca H., Öztürk A. A. (Executive)

Project Supported by Higher Education Institutions, 2018 - 2019

  • Project Type: Project Supported by Higher Education Institutions
  • Begin Date: May 2018
  • End Date: July 2019

Project Abstract

Abstract As it is known, the existence of diseases on earth is based on ancient histories as the existence of human beings, and from the earliest times until day by day all people tried to treat these diseases by using various substances. For this purpose, while the various extracts obtained from plants and animals were used for the first time, the progress of science and the discovery of new substances, especially antibiotics, resulted in drugs which were considered to be miraculous. Infectious diseases are an area where it has been achieved with antimicrobial treatment that is important and appropriate in terms of public health. Nevertheless, infectious diseases that are acquired both in the community and in the hospital can not be treated as a result of the use of unreasonable antimicrobial treatments, and perhaps the patient is lost. Appropriate antimicrobial therapy; survival, complications and prevention of chronicity are important in terms of severity and duration of disease. For ideal antibiotic use; correct antibiotic after diagnosis; the most appropriate route, the effective dose, the optimum intervals, the appropriate time should be given. Antibiotic resistance has become an important health problem all over the world. Infections caused by antibiotic-resistant bacteria result in increased disease and mortality rates, prolonged hospital stay, and also an increase in treatment costs. The most important reason for antibiotic resistance is the high doses of antibiotic drugs in the market and the use of high doses. In addition, in today's drug therapy, the patient is exposed several times a day and in high doses. High doses have serious side effects with it. Clarithromycin is a semisynthetic macrolide derivative antibiotic. It inhibits protein synthesis by binding to the ribosomal subunit of the 50S of susceptible bacteria and thus exhibits antibacterial activity. Clarithromycin is active against many gram positive and gram negative bacterial. Clarithromycin is administered to the patient more than twice a day. Excessive dosing may increase toxic effects, as well as patient forgetfulness of dosing. The polymeric nanoparticulate system is a drug-delivery system that has been intensively studied, controlled and sustained release over the last few years. In this work, the prospective clarithromycin-loaded polymeric nanoparticle system PLGA (Poly Lactic-co-Glycolic Acid) polymer will be prepared by nano-precipitation method to prolong release of active substance and increase antimicrobial activity. Once the optimal formulation has been determined, the PLGA nanoparticle will be coated with a natural polymer, chitosan, which has spontaneous antimicrobial activity. Scope of work; development of formulations, in vitro characterization, short-term stability study to be performed in gastrointestinal fluids, and determination of the antimicrobial effect profile of the optimum formulation selected. Prepared formulations are expected to create a new approach in antimicrobial therapy.