DPPH Scavenging, PRAP Activities and Essential Oil Composition of Edible Lathyrus Ochrus L. (Cyprus Vetch, Luvana) from Cyprus

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Polatoğlu K., Arsal S., DEMİRCİ B., Can Başer K. H.

Journal of Oleo Science, cilt.64, sa.3, ss.309-314, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 3
  • Basım Tarihi: 2015
  • Doi Numarası: 10.5650/jos.ess14173
  • Dergi Adı: Journal of Oleo Science
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.309-314
  • Anahtar Kelimeler: Lathyrus ochrus, essential Oil, HPTLC-DPPH assay, HPTLC-PRAP assay, phytol, VOLATILE CONSTITUENTS, CHEMICAL-COMPOSITION, PHYTANIC ACID, FLOWER, PHYTOL, SEEDS
  • Anadolu Üniversitesi Adresli: Evet

Özet

© 2015 by Japan Oil Chemists‘ Society.The essential oil of the aerial parts of edible Lathyrus ochrus L. was investigated by simultaneous GC, GC/MS analyses under the same conditions. Trace amount of oil (0.01> mL) obtained by hydro distillation of 200 g fresh plants was trapped in 1 mL n-hexane. Twenty components were detected representing 91.55 ± 0.56% of the oil. The main components were phytol 49.39 ± 0.44%, hexadecanoic acid 20.64 ± 0.89% and pentacosane 4.20 ± 0.09%. Essential oil solution (1% oil: n-hexane) afforded similar DPPH scavenging activity (9.28 ± 1.30%) when compared with positive controls α-tocopherol (9.74 ± 0.21%) and BHT (7.79 ± 0.26%) at the same concentrations. Antioxidant activity of the oil was determined using a new HPTLC-PRAP assay. The oil afforded two fold higher reducing activity of phosphomolybdenum complex (594.85 ± 5.14 AU) when compared with positive controls α- tocopherol (271.10 ± 2.86 AU) and BHT (210.53 ± 1.81 AU) at the same concentration.