Potential Recruiting and Hepatoprotective Effects of Ellagic Acid in D-Galactosamine-Induced Liver Damage in Rats


Cengiz M., Ali J. H., KUTLU H. M., Vejselova D., AYHANCİ A.

PAKISTAN JOURNAL OF ZOOLOGY, vol.49, no.4, pp.1251-1259, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 49 Issue: 4
  • Publication Date: 2017
  • Doi Number: 10.17582/journal.pjz/2017.49.4.1251.1259
  • Journal Name: PAKISTAN JOURNAL OF ZOOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1251-1259
  • Keywords: D-GaIN, Ellagic acid, Hepatotoxicity, TEM, Rats, VITAMIN-E SUCCINATE, OXIDATIVE STRESS, CARBON-TETRACHLORIDE, HEPATITIS, MECHANISM, ANTIOXIDANT, APOPTOSIS, INJURY, MICE, HEPATOTOXICITY
  • Anadolu University Affiliated: Yes

Abstract

The present study aims to investigate the therapeutic and protective effects of ellagic acid (EA) on the toxicity of the liver induced by D-Galactosamine (D-GaIN) in rats. With this in mind, the rats were categorized into five groups. The study groups were given saline, 0.2 % dimethyl sulfoxide, D-GaIN, EA plus D-GaIN and D-GaIN plus EA, respectively. In the group given D-GaIN, the following transmission electron microscopic and light microscopic results were found: degenerative changes in the liver tissue, significant decreased in the number of activated Bcl-2, while increased in the number of Bax and caspase-3-positive hepatocytes, a significantly increase in levels of the activities of biochemistry markers (serum alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP)). In contrast, in the groups given D-GaIN and EA, a decrease in the damage of the liver tissue, a significant decrease activated Bax and caspase-3-positive hepatocytes, while increase in the number of Bcl-2 positive hepatocytes, a decrease in the biochemistry markers levels were found. Group 4, given EA before D-GaIN, showed better results when compared to Group 5, given EA after D-GaIN, in terms of histopathological and biochemical values. In conclusion, EA might play an important role in repairing D-GaIN-induced liver damage both as a protective and a therapeutic agent.