Vincristine and ε-viniferine-loaded PLGA-b-PEG nanoparticles: pharmaceutical characteristics, cellular uptake and cytotoxicity

Ogunc Y., DEMİREL M., YAKAR A., Incesu Z.

JOURNAL OF MICROENCAPSULATION, sa.1, ss.38-46, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/02652048.2017.1282549
  • Dergi Adı: JOURNAL OF MICROENCAPSULATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.38-46
  • Anahtar Kelimeler: Nanoparticle, PLGA-b-PEG, passive targeting, epsilon-viniferine, vincristine, DRUG-DELIVERY, IN-VITRO, INDUCED APOPTOSIS, PARTICLE-SIZE, CANCER-CELLS, FOLIC-ACID, RED WINE, RESVERATROL, THERAPY, SULFATE
  • Anadolu Üniversitesi Adresli: Evet

Özet

The objective of this study was to prepare the epsilon-viniferine and vincristine-loaded PLGA-b-PEG nanoparticle and to investigate advantages of these formulations on the cytotoxicity of HepG2 cells. Prepared nanoparticle has shown a homogeneous distribution with 113 +/- 0.43nm particle size and 0.323 +/- 0.01 polydispersity index. Zeta potential was determined as -35.03 +/- 1.0mV. The drug-loading percentages were 6.01 +/- 0.23 and 2.01 +/- 0.07 for epsilon-viniferine and vincristine, respectively. The cellular uptake efficiency of coumarin-6-loaded nanoparticles was increased up to 87.8% after 4h. Nanoparticles loaded with high concentrations of both drugs showed a cytotoxic effect on HepG2 cells, having the percentage of cell viability of between 43.23% and 47.37%. Unfortunately, the percentage of apoptotic cells after treated with drugs-loaded nanaoparticles (10.93%) was similar to free forms of drugs (12.1%) that might be due to low epsilon-viniferine release in biological pH at 24h.