Phytochemical profiling and evaluation of marrubium sivasense aytaç, akgül & ekici for antioxidant activity and inhibition effects on α-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes

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Göger F., ÖZEK G., TEKİN M., Yur S., ÖZEK T.

Journal of the Turkish Chemical Society, Section A: Chemistry, cilt.6, sa.3, ss.281-292, 2019 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 3
  • Basım Tarihi: 2019
  • Doi Numarası: 10.18596/jotcsa.516888
  • Dergi Adı: Journal of the Turkish Chemical Society, Section A: Chemistry
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.281-292
  • Anahtar Kelimeler: Antioxidant, Extract, LC-MS/MS, Marrubium sivasense, Tyrosinase, Xanthine oxidase, α-amylase
  • Anadolu Üniversitesi Adresli: Evet

Özet

© 2019, Turkish Chemical Society. All rights reserved.In the present study, the extract of an endemic plant ourkey Marrubium sivasense Aytaç, Akgül & Ekici was investigated for phytochemical profile, antioxidant properties and inhibition of several enzymes. The chemical composition of the extract was investigated with LC-MS/MS technique. Forsythoside B, verbascoside and leucoseptoside were identified as the main compounds. The free radical scavenging activity of the extract was determined against DPPH• radicals (IC50 0.34 mg/mL) and ABTS•+ cation radicals (TEAC 2.3 mM). In the xanthine-xanthine oxidase (XO) system and in β-carotene bleaching assay the extract demonstrated moderate activity (Inh% 16.54 and Inh% 43±1.26, respectively). The extract demonstrated hypoglycemic activity (Inh% 49.67±1.09) via inhibition of porcine pancreatic α-amylase. The antiinflammatory effect tested via inhibition of 5-LOX was found as Inh% 18.71±0.74. However, no antityrosinase activity was found.