Phytochemical profiling and evaluation of marrubium sivasense aytaç, akgül & ekici for antioxidant activity and inhibition effects on α-amylase, lipoxygenase, xanthine oxidase and tyrosinase enzymes
Journal of the Turkish Chemical Society, Section A: Chemistry, cilt.6, sa.3, ss.281-292, 2019 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 6 Sayı: 3
- Basım Tarihi: 2019
- Doi Numarası: 10.18596/jotcsa.516888
- Dergi Adı: Journal of the Turkish Chemical Society, Section A: Chemistry
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.281-292
- Anahtar Kelimeler: Antioxidant, Extract, LC-MS/MS, Marrubium sivasense, Tyrosinase, Xanthine oxidase, α-amylase
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Anadolu Üniversitesi Adresli: Evet
Özet
© 2019, Turkish Chemical Society. All rights reserved.In the present study, the extract of an endemic plant ourkey Marrubium sivasense Aytaç, Akgül & Ekici was investigated for phytochemical profile, antioxidant properties and inhibition of several enzymes. The chemical composition of the extract was investigated with LC-MS/MS technique. Forsythoside B, verbascoside and leucoseptoside were identified as the main compounds. The free radical scavenging activity of the extract was determined against DPPH• radicals (IC50 0.34 mg/mL) and ABTS•+ cation radicals (TEAC 2.3 mM). In the xanthine-xanthine oxidase (XO) system and in β-carotene bleaching assay the extract demonstrated moderate activity (Inh% 16.54 and Inh% 43±1.26, respectively). The extract demonstrated hypoglycemic activity (Inh% 49.67±1.09) via inhibition of porcine pancreatic α-amylase. The antiinflammatory effect tested via inhibition of 5-LOX was found as Inh% 18.71±0.74. However, no antityrosinase activity was found.