Isolation and identification of selenite reducing archaea from Tuz (salt) Lake In Turkey

GÜVEN K., MUTLU M. B., Cirpan C., KUTLU H. M.

JOURNAL OF BASIC MICROBIOLOGY, vol.53, no.5, pp.397-401, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 53 Issue: 5
  • Publication Date: 2013
  • Doi Number: 10.1002/jobm.201200008
  • Journal Name: JOURNAL OF BASIC MICROBIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.397-401
  • Keywords: Selenite, Archaea, Tuz Lake, TEM, Halorubrum sp, DE-FUCA RIDGE, RESISTANT BACTERIA, ELEMENTAL SELENIUM, HYDROTHERMAL VENTS, REDUCTION, SELENATE, TOLERANCE, NANOWIRES, OXYANIONS, FEATURES
  • Anadolu University Affiliated: Yes

Abstract

In this study, Tuz lake brine samples were investigated for isolation and identification of selenite resistant halophilic prokaryotes. Among the 20 strains of extremely halophilic Bacteria and Archaea, a Gram negative rod designated as strain 106, showed high capacity in the resistance to selenite (25mM) under aerobic conditions. Phenotypic characterizations and phylogenetic analyses based on 16S rDNA sequence comparison indicated that strain 106 was Halorubrum xinjiangense. The ability of strain 106 to deposite selenium-containing particles were investigated by Transmission Electron Microscopy (TEM). Electron micrographs shows intact cells after selenite reduction and large amounts of selenium-containing particles are present in the culture medium indicating that strain 106 is able to efficiently transport elemental selenium out of the cell.