Genotoxicity of aspartame

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Rencuzogullari E., Tuylu B., Topaktas M., Ila H., Kayraldiz A., Arslan M., ...More

DRUG AND CHEMICAL TOXICOLOGY, vol.27, no.3, pp.257-268, 2004 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 3
  • Publication Date: 2004
  • Doi Number: 10.1081/dct-120037506
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.257-268
  • Keywords: sweeteners, aspartame, chromosome aberrations, sister chromatid exchange, micronuclei, ames test, BREAST-CANCER, SWEETENERS, MELPHALAN, RISK
  • Anadolu University Affiliated: No


In the present study, the genotoxic effects of the low-calorie sweetener aspartame (ASP), which is a dipeptide derivative, was investigated using chromosome aberration (CA) test, sister chromatid exchange (SCE) test, micronucleus test in human lymphocytes and also Ames/Salmonella/ microsome test. ASP induced CAs at all concentrations (500, 1000 and 2000 mug/ml) and treatment periods (24 and 48 h) dose-dependently, while it did not induce SCEs. On. the other hand, ASP decreased the replication index (RI) only at the highest concentration for 48 h treatment period. However, ASP decreased the mitotic index (MI) at all concentrations and treatment periods dose-dependently. In addition, ASP induced micronuclei at the highest concentrations only. This induction was also dose-dependent for 48 hours treatment period. ASP was not mutagenic for Salmonella typhimurium TA98 and TA 100 strains in the absence and presence of S9 mix.