Ketorolac tromethamine (KT) is a non-steroidal anti-inflammatory drug (NSAID) from the heteroaryl acetic acid derivatives class that acts as a non-selective COX inhibitor. KT is often used successfully in post-surgical (postoperative) pain and surgical wounds [1]. The most widely used new nanotechnological approaches for topical drug delivery are nanoparticles (NPs) prepared with natural polymers, polymeric NPs, nano-emulsions, lipid-based nanoparticles, metal nanoparticles and dendrimers [2]. Spray drying is one of the most extensively studied processes in the pharmaceutical field. It is frequently used for drug formulations to be used topically [3]. Fibre-forming collagen type I, encoded by the COL1A1 gene, is the most common collagen in the skin, accounting for 90% of the total. Systemic organization of tissue is crucial for wound healing as it is vital to its integrity and strength [4]. If the physiologically in- flammatory response in wound healing is prolonged or exacerbated, it leads to a delay in the later stages of proper wound healing. In this case, anti-inflammatory agents are needed [5]. Within the scope of this study, KT loaded nanoparticle-based drug delivery systems, which can be effective in pain treatment and wound healing processes in postoperative conditions, were prepared. After the characterization procedures, the cytotoxicity of the optimum formulation in human fibroblast cells was determined by the WST- 1 method and the gene activity was elucidated by mRNA isolation and real-time polymerase chain reaction (RT-PCR).