Research Information System
15th International Congress of Histochemistry and Cytochemistry, Antalya, Turkey, 18 - 21 May 2017, pp.1
INTRODUCTION AND OBJECTIVES
The aim of the study was to investigate the potential cytotoxic effects of silymarin loaded solid lipid nanoparticle on MCF7 cells and its effects on MCF7 cell ultrastructure.
MATERIAL AND METHODS
The cytotoxic effects of nano-silymarin formulation on MCF7 cells were determined by MTT colorimetric assay. Stock solution of commercial silymarin (in DMSO), was applied to MCF7 cells for 24 hours in different concentrations. The plates were read on ELISA reader at 570nm wavelength. For detecting the morphological changes, MCF7 cells were treated with the IC50 concentration of silymarin-nano form for 24 hours. After incubation, cells were stained with Alexa fluor-488 phalloidin and acridine orange and evaluated and photographed under confocal microscope and transmission microscope.
RESULTS
Viability percentages of silymarin-nano treated cells decreased in low drug concentrations and lC50 (18µM) value was calculated. This value altered the morphology of MCF7 cells causing disintegrated and deformed nuclei, cell skeleton and chromatin, holes on cytoskeleton as well as cell shrinkage.
MATERIAL AND METHODS
The cytotoxic effects of nano-silymarin formulation on MCF7 cells were determined by MTT colorimetric assay. Stock solution of commercial silymarin (in DMSO), was applied to MCF7 cells for 24 hours in different concentrations. The plates were read on ELISA reader at 570nm wavelength. For detecting the morphological changes, MCF7 cells were treated with the IC50 concentration of silymarin-nano form for 24 hours. After incubation, cells were stained with Alexa fluor-488 phalloidin and acridine orange and evaluated and photographed under confocal microscope and transmission microscope.
CONCLUSIONS
On the basis on our results, it might be concluded that silymarin-nano formulation form showed high cytotoxicity on human breast cancer cells and exerts a potential promising to be cytotoxic and anti-proliferative as well apoptosis triggering agent in cancer cells for design of pharmaceutical products but further deeper investigations are required.
KEYWORDS: Silymarin, Nano-compound, MCF7, Confocal microscopy, TEM.