Determination of formoterol by capillary electrophoresis and its application to inhaler capsules

ŞENER E., Tuncel M., Aboul-Enein H.

ARCHIV DER PHARMAZIE, cilt.336, sa.4-5, ss.226-229, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 336 Sayı: 4-5
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1002/ardp.200300733
  • Dergi Adı: ARCHIV DER PHARMAZIE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.226-229
  • Anahtar Kelimeler: formoterol, capillary electrophoresis, pharmaceutical analysis, inhaler capsule, PERFORMANCE LIQUID-CHROMATOGRAPHY, ELECTROCHEMICAL DETECTION, ENANTIOMERS, ASSAY, SEPARATION, FUMARATE, AGONISM, POTENT, DRUGS
  • Anadolu Üniversitesi Adresli: Evet

Özet

A capillary electrophoretic (CE) method for the determination of formoterol (FOR) in a pharmaceutical preparation is described. Analysis was made in a background electrolyte consisting of 20 % acetonitrile and 50 mM phosphoric acid at pH 2.5, using fused silica capillary (86 cm x 75 pm ID), 27 kV potential, and detecting at 200 nm. Under these electrophoretic conditions 3,4-dihydroxybenzylamine used as an internal standard (IS) and FOR showed symmetrical peaks at 6.1 and 8.3 min., respectively. The inter-day and intra-day precision was examined in the concentration range of 2.98 x 10(-6) V M to 8.94 x 10(-6) M. Good correlation and accuracy were obtained. Limit of detection, (LOD) and limit of quantitation (LOQ) values were 3.71 x 10(-7) M and 1.11 x 10(-6) M, respectively The method was applied for the analysis of FOR in pharmaceutical inhaler capsules. The proposed method is reliable, precise, accurate, fast, and cost effective.