Primary cilium in CO25 cell line

Zeytinoglu M., Ocak H.

15th Annual Meeting of the Japanese-Association-for-Animal-Cell-Technology, Fuchu, Japan, 11 - 15 November 2002, pp.493-497 identifier

  • Publication Type: Conference Paper / Full Text
  • City: Fuchu
  • Country: Japan
  • Page Numbers: pp.493-497
  • Keywords: primary cilium, myoblast, microtubule, Tyr-tubulin, Glu-tubulin, MYOGENIC CELLS, IN-VITRO, DIFFERENTIATION, 3T3-CELLS, TUBULIN
  • Anadolu University Affiliated: No


A few variety of cell type contain a primary cilium. Also the biological function(s) of primary cilia within such cell types are largely unknown. CO25 myoblast cell lines were cultured then they were stained with anti Glu- and Tyr- tubulin in this study. Glu-tubulin rich microtubules were only rarely seen in the cells. But the primary cilia was strongly stained by anti Glu-tubulin and also they were usually showed some weak staining with the anti Tyr-tubulin stain. Usually the differentiating myoblast cells had one primary cilium which has always localized over the top of the nuclei. The primary cilia always had a wider base and the botom the shaft was thicker than the upper part. Cytoplasmic microtubules have reorganized and also primary cilium has formed after replacing of centrioles in progression of myogenesis.