A new potentiometric platform: Antibody cross-linked graphene oxide potentiometric immunosensor for clenbuterol determination

BİRLİK ÖZKÜTÜK E., ERSÖZ A., Ugurag D., Yilmaz F., Say R.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, vol.68, no.6, pp.1153-1158, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 6
  • Publication Date: 2021
  • Doi Number: 10.1002/bab.2036
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Applied Science & Technology Source, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1153-1158
  • Keywords: antibody cross-linked, clenbuterol, potentiometric sensor, PERFORMANCE LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, ELECTRODES, BEEF
  • Anadolu University Affiliated: Yes


An immunosensor is based on the signal measurement obtained upon the reaction of an antibody antigen complex. It plays a significant role in various fields such as environmental analysis, production monitoring, drug detection or screening, veterinary medicine, and animal food. In this study, an antibody crosslinked graphen oxide (GO)-based potentiometric sensor has been developed for fast, simple, and economical detection of clenbuterol. In this context, the photosensitive amino acid bound GO platform is synthesized and used for the preparation of electrode material. Then, polymeric structure is characterized by infrared spectroscopy, and the performance of immunonano platform prepared by potentiometric sensor is evaluated. The effect of pH, response time, selectivity, and sensitivity is investigated. Under the optimized conditions, a simple and rapid method for the determination of clenbuterol from milk sample is established by immuno-potentiometric sensor. The detection limit has found to be 0.87 x 10(-9)mmol L(-1)for this immuno-potentiometric sensor. This immuno-potentiometric sensor has optimum pH at 7.0, a wide linear response (1.0 x 10(-2)to 1.0 x 10(-9)mmol L-1), rapid response time (2 Min) and 36 weeks operational lifetime.