Development of a disposable and low-cost electrochemical sensor for dopamine detection based on poly(pyrrole-3-carboxylic acid)-modified electrochemically over-oxidized pencil graphite electrode

Ozcan A., Ilkbas S., Ozcan A. A.

TALANTA, vol.165, pp.489-495, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 165
  • Publication Date: 2017
  • Doi Number: 10.1016/j.talanta.2017.01.007
  • Journal Name: TALANTA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.489-495
  • Keywords: Dopamine, Poly(pyrrole-3-carboxylic acid), Electrochemical over-oxidation, Modified electrodes, Sensor, SENSITIVE VOLTAMMETRIC DETERMINATION, URIC-ACID, LIQUID-CHROMATOGRAPHY, MASS-SPECTROMETRY, ASCORBIC-ACID, BLOOD-SERUM, FABRICATION, OXIDATION, QUANTIFICATION, SEROTONIN
  • Anadolu University Affiliated: Yes

Abstract

In this study, preparation of a single-use electrochemical sensor for the selective and sensitive determination of dopamine (DOP) was investigated by electrochemical polymerization of pyrrole-3-carboxylic acid on electrochemically over-oxidized pencil graphite electrode (p(P3CA)/EOPGE). Cyclic voltammetry measurements of Fe(CN)(6)(4-/3-) indicated that the electrochemically over-oxidized PGE (EOPGE) showed superior electron transfer characteristics according to bare PGE. The ionized carboxyl groups found in the structure of poly(pyrrole-3-carboxylic acid) (p(P3CA)) showed high affinity towards positively charged DOP. The combination of the advantages of EOPGE and p(P3CA) in p(P3CA)/EOPGE led to a synergistic effect on the electrochemical oxidation of DOP. The effects of experimental variables on the voltammetric performance of the p(P3CA)/EOPGE were examined by preparing the electrodes at different conditions. The p(P3CA)/EOPGE showed high selectivity towards DOP by discriminating its oxidation potential from the common interfering substances such as ascorbic and uric acids. The p(P3CA)/EOPGE showed linear responses in the electrochemical oxidation of DOP between the concentration values of 0.025 mu M and 7.5 mu M. Detection limit was determined as 0.0025 mu M according to signal to noise ratio (S/N: 3). Analytical application of p(P3CA)/EOPGE was successfully tested in the determination of DOP in blood serum and pharmaceutical samples.