In this study, preparation of a single-use electrochemical sensor for the selective and sensitive determination of dopamine (DOP) was investigated by electrochemical polymerization of pyrrole-3-carboxylic acid on electrochemically over-oxidized pencil graphite electrode (p(P3CA)/EOPGE). Cyclic voltammetry measurements of Fe(CN)(6)(4-/3-) indicated that the electrochemically over-oxidized PGE (EOPGE) showed superior electron transfer characteristics according to bare PGE. The ionized carboxyl groups found in the structure of poly(pyrrole-3-carboxylic acid) (p(P3CA)) showed high affinity towards positively charged DOP. The combination of the advantages of EOPGE and p(P3CA) in p(P3CA)/EOPGE led to a synergistic effect on the electrochemical oxidation of DOP. The effects of experimental variables on the voltammetric performance of the p(P3CA)/EOPGE were examined by preparing the electrodes at different conditions. The p(P3CA)/EOPGE showed high selectivity towards DOP by discriminating its oxidation potential from the common interfering substances such as ascorbic and uric acids. The p(P3CA)/EOPGE showed linear responses in the electrochemical oxidation of DOP between the concentration values of 0.025 mu M and 7.5 mu M. Detection limit was determined as 0.0025 mu M according to signal to noise ratio (S/N: 3). Analytical application of p(P3CA)/EOPGE was successfully tested in the determination of DOP in blood serum and pharmaceutical samples.