In vitro preliminary studies of chitooligosaccharide coated nanostructured lipidic nanoparticles for efficient gene delivery

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JOURNAL OF RESEARCH IN PHARMACY, vol.23, no.4, pp.671-681, 2019 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 4
  • Publication Date: 2019
  • Doi Number: 10.12991/jrp.2019.175
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.671-681
  • Keywords: Chitooligosaccharide lactate, lipidic nanocarrier, gene delivery, cytotoxicity, transfection, PARTICLE-SIZE, STABILITY, CARRIERS, SYSTEMS, NLC, OLIGOCHITOSAN, SLN
  • Anadolu University Affiliated: Yes


Gene therapy is generally defined as the transfer of genetic material to cells to treat a disease or at least improve the clinical condition of a patient. The most commonly used vectors in gene therapy are viral and non-viral vectors. The aim of this study was to develop a gene carrier system based on chitooligosaccharide-coated nanostructured lipidic nanoparticles and evaluate the physicochemical properties, such as zeta potential, particle size, SEM images, pH, cytotoxicity, DNA-binding properties, serum stability and transfection to cells. In this study, cationic formulations were produced using Dynasan (R) 116 and Transcutol (R) p as a lipidic phase and chitooligosaccharide lactate for polymeric coating with DOTAP as a cationic agent. These formulations were made with the oil-in water hot emulsification technique. GFP was selected as the genetic material to be loaded into the formulations. According to the results, the chitooligosaccharide-coated cationic lipid nanoparticles prepared had considerably small particle sizes (144-178 nm) and high zeta potential (+37.6/+33.7mV). Based on the MTT assay, the cytotoxic effect of formulations on the NIH 3T3, A549 and MDA-MB-231 cell lines exhibited a dose-time-dependant pattern. Further, the prepared formulations binded DNA effectively and protected DNA against the serum component. It was concluded that chitooligosaccharide-coated lipidic nanoparticle formulations can be prepared as a pDNA-nanoparticle complex and can be employed as a gene delivery system effectively.