An LC Method for the Determination of Bupropion and Its Main Metabolite, Hydroxybupropion in Human Plasma

YENİCELİ UĞUR D., Dogrukol-Ak D.

CHROMATOGRAPHIA, no.11-12, pp.1703-1708, 2009 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2009
  • Doi Number: 10.1365/s10337-009-1361-z
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1703-1708
  • Keywords: Column liquid chromatography, Protein precipitation, Bupropion and hydroxybupropion in human plasma, SUSTAINED-RELEASE BUPROPION, LIQUID-CHROMATOGRAPHY, SMOKING-CESSATION, MAJOR METABOLITES, PHARMACOKINETICS, SEPARATION, COLUMN, HYDROCHLORIDE
  • Anadolu University Affiliated: Yes


A new LC method has been developed and validated for the direct determination of bupropion and its main metabolite, hydroxybupropion in human plasma. Plasma samples were analyzed after a simple, one step protein precipitation with trichloroacetic acid using a C-8 column and mobile phase, consisting of methanol/acetonitrile/phosphate buffer (10 mM, pH 3.0) (40:10:50, v/v/v) and 20 mM 1-heptane sulfonic acid sodium salt with carbamazepine as the internal standard. UV detection was performed at 214 and 254 nm. The method was validated over the concentration range of 60-2,400 and 150-4,700 ng mL(-1) for bupropion and hydroxybupropion, respectively. The intra- and inter-day assay variability was less than 15% for the two analytes. Limit of detection values were 24.8 and 63.4 ng mL(-1) for bupropion and hydroxybupropion, respectively. The method developed was applied to quantification of bupropion and hydroxybupropion in human plasma.