A new approach for the construction of dual character in nanosystems

Kose E., Say R., ERSÖZ A.

SENSORS AND ACTUATORS B-CHEMICAL, vol.222, pp.1012-1017, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 222
  • Publication Date: 2016
  • Doi Number: 10.1016/j.snb.2015.09.019
  • Journal Name: SENSORS AND ACTUATORS B-CHEMICAL
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1012-1017
  • Keywords: Dual character nanoparticles, Nanoenzyme, Horseradish peroxidase, Alkaline phosphatase, Photosensitive monomer, ANADOLUCA, HORSERADISH-PEROXIDASE, CEPACIA LIPASE, CANDIDA-RUGOSA, REMOVAL, CHLOROPHENOL, PARTICLES, CHEMISTRY, SUBSTRATE, SILICA, WATER
  • Anadolu University Affiliated: Yes

Abstract

This study describes the preparation of nanoprotein particles carrying horseradish peroxidase (HRP) and alkaline phosphatase (ALP) using a photosensitive microemulsion polymerization technique. The prepared nanoparticles having dual character were characterized by transmission electron microscopy (TEM) and Zeta Sizer. The average particle size of p(HRP-co-ALP) nanoparticles was found to be about 90 nm. The activity of nanoparticles having dual character was assayed using tetramethylbenzidine and paranitrophenyl phosphate (p-NPP) as substrates for HRP and ALP, respectively. The parameters such as pH, temperature and initial enzyme concentration that affect the activity were investigated by using p(HRP-co-ALP) nanoparticles and compared with the free HRP and ALP. The reusability of p(HRP-co-ALP) particles was also investigated and the results showed that the prepared nanoparticles exhibited admirable potential as a reusable catalyst. (C) 2015 Elsevier B.V. All rights reserved.