Synthesis of 5-(4-methacrylamidophenyl)-10,15,20-triphenylporphyrin, its copolymerization with acrylamide and EDMA, use of this copolymer in the adsorption of bovine serum albumin

Tay T., Tuerk H., Say R.

REACTIVE & FUNCTIONAL POLYMERS, vol.67, no.10, pp.999-1007, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 10
  • Publication Date: 2007
  • Doi Number: 10.1016/j.reactfunctpolym.2007.06.003
  • Journal Name: REACTIVE & FUNCTIONAL POLYMERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.999-1007
  • Keywords: adsorption, bovine serum albumin, porphyrin, hydrophobic interaction, WATER-SOLUBLE PORPHYRINS, HYDROPHOBIC INTERACTION CHROMATOGRAPHY, AFFINITY CAPILLARY-ELECTROPHORESIS, FUNCTIONAL METALLOMACROCYCLES, AMINO-ACIDS, BINDING, PROTEINS, COMPLEX, DNA, AUTOXIDATION
  • Anadolu University Affiliated: No

Abstract

A rigid and porous polymeric adsorbent was prepared from monomers 5-(4-methacrylamidophenyl)-10,15,20-triphenylporphyrin (1%), acrylamide (39%), and cross-linking agent ethylene glycol dimethacrylate (60%) using suspension polymerization technique. The adsorption behavior of bovine serum albumin (BSA) on this adsorbent was studied as a function of the initial BSA concentration (0.4-2.5 mg/mL), pH (4.0-8.0), ionic strength, and type of salt in buffered solutions under batch equilibrium conditions. The amounts of adsorbed BSA at equilibrium at pH 4.0 and 7.0 for 1.0 mg/mL initial concentration of BSA were 743 mg BSA and 412 mg BSA per gram of the adsorbent, respectively. The adsorption process was nearly irreversible. (c) 2007 Elsevier Ltd. All rights reserved.