HPTLC-DPPH center dot and HPTLC-tyrosinase methods for hot water-soluble contents of kumquat, limequat and Mexican lime fruit powders

AĞALAR H. G., TEMİZ B.

JOURNAL OF RESEARCH IN PHARMACY, vol.25, no.5, pp.569-580, 2021 (ESCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 5
  • Publication Date: 2021
  • Doi Number: 10.29228/jrp.48
  • Journal Name: JOURNAL OF RESEARCH IN PHARMACY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.569-580
  • Keywords: kumquat, limequat, Mexican lime, HPTLC-DPPH center dot assay, HPTLC-tyrosinase assay, phloretin-di-C-glucoside, ANTIOXIDANT ACTIVITY, VOLATILE COMPOUNDS, CITRUS, PEEL, EXTRACTS, OIL, INHIBITION, COMPONENTS, SWINGLE, MS
  • Anadolu University Affiliated: Yes

Abstract

In the present study, small Citrus fruits which are cultivated in our country and have an economic value were chosen as research materials. It was aimed to do fingerprint analysis of the fruits by HPTLC method. DPPH center dot scavenging and antityrosinase potentials were determined in vitro. Extracts showed noticable activities, but these effects were not comparable with positive controls. HPTLC-bioautographic assays and Mass Spectrometry analysis were employed to identify bioactive compound in the extracts. Phloretin-di-C-glucoside was tentatively identified as DPPH center dot scavenging and antityrosinase active compound in kumquat and limequat extracts.