Inhibition effects of pesticides on glutathione-S-transferase enzyme activity of Van Lake fish liver

Ozaslan M. S., Demir Y., AKSOY M., KÜFREVİOĞLU Ö. İ., BEYDEMİR Ş.

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, vol.32, no.9, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 32 Issue: 9
  • Publication Date: 2018
  • Doi Number: 10.1002/jbt.22196
  • Journal Name: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: glutathione-S-transferase, inhibition, pesticides, purification, Van Lake fish, CHALCALBURNUS-TARICHII PALLAS, CARBONIC-ANHYDRASE ACTIVITY, ACUTE EXPOSURE, PURIFICATION, HISTOPATHOLOGY, CHLORPYRIFOS, RESPONSES, TISSUES, GILL
  • Anadolu University Affiliated: Yes

Abstract

Glutathione-S-transferases (GSTs) have a function in xenobiotic metabolism. They are a significant multifunctional family with a wide variety of catalytic activities. In the current study, we determined in vitro inhibition effects of 2,4-dichlorophenoxyacetic acid dimethylamine salt (2,4-D DMA), haloxyfop-P-methyl, glyphosate isopropylamine, dichlorvos, and -cyhalothrin on purified GST. For this purpose, GST were purified from Van Lake fish (Chalcalburnus tarichii Pallas) liver with 29.25 EUmg(-1) specific activity and 10.76% yield using GSH-agarose affinity chromatographic method. The pesticides were tested at various concentrations on in vitro GST activity. K-i constants were calculated as 0.17 +/- 0.01, 0.25 +/- 0.05, 3.72 +/- 0.32, 0.42 +/- 0.06, and 0.025 +/- 0.004mM, for 2,4-D DMA, haloxyfop-P-methyl, glyphosate isopropylamine, dichlorvos, and -cyhalothrin, respectively. -Cyhalothrin showed a better inhibitory effect compared to the other pesticides. The inhibition mechanisms of -cyhalothrin were competitive, while the other pesticides were noncompetitive.