Determination of the anti-inflammatory activity of nano spray-dried nanoparticles with in vivo HET-CAM assay that can be used in post-operative wound healing

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Öztürk A. A., Çevikelli T., Güven U. M., Kıyan H. T.

International Congress on Biological and Health Science, İstanbul, Turkey, 24 - 27 February 2022, pp.1-6

  • Publication Type: Conference Paper / Full Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.1-6
  • Anadolu University Affiliated: Yes


Ketorolac tromethamine is a non-steroidal anti-inflammatory drug (NSAID) from the heteroaryl acetic acid derivatives class that acts as a non-selective COX inhibitor. KT is often used successfully in post-surgical (postoperative) pain and surgical wounds. If the physiologically inflammatory response in wound healing is prolonged or exacerbated, it leads to a delay in the later stages of proper wound healing. In this case, anti-inflammatory agents are needed. Spray drying is one of the most extensively studied processes in the pharmaceutical field. It is frequently used for drug formulations to be used topically. Within the scope of this study, ketorolac tromethamine loaded nanoparticle based drug delivery systems, which can be effective in pain treatment and wound healing processes in postoperative conditions, were prepared. The in vivo ‘Hen's Egg Test on the Chorioallantoic Membrane’ assay was performed on the selected optimum formulation for anti-inflammatory activity. The in vivo anti- inflammatory activity results indicated that the nanoparticle formulation including ketorolac tromethamine as 100 μg / pellet showed significantly strong anti-inflammatory potential with 85.625 ± 11.825 % inhibition value and no embryotoxicity whereas ketorolac tromethamine showed weak activity with 60 ± 13.675 % inhibition on the chorioallantoic Membrane. With the obtained Hen's Egg Test on the chorioallantoic Membrane assay result, it has the hope of a successful nano-topical formulation especially in wound healing.