Rapid HPLC and direct flow injection analysis assay for the determination of trimetazidine HCl in pharmaceutical tablet formulation

Altiokka G., Kircali K., Aboul-Enein H. Y.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.29, sa.15, ss.2245-2255, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 15
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1080/10826070600832921
  • Dergi Adı: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2245-2255
  • Anahtar Kelimeler: trimetazidine HCl, high performance liquid chromatography, flow injection analysis, pharmaceutical analysis, SPECTROPHOTOMETRIC DETERMINATION, DIHYDROCHLORIDE, DRUG
  • Anadolu Üniversitesi Adresli: Hayır

Özet

A simple and sensitive high performance liquid chromatographic method (HPLC) and direct flow injection analysis method (FIA) are described for the determination of trimetazidine HCl (TMZ) in tablets. A mobile phase consisting of methanol:0.02 mol center dot L -1 potassium dihydrogen phosphate:0.005 mol center dot L -1 sodium dihydrogen phosphate (62:5:33 v/v/v) was used for the resolution of the compound on a reversed phase column for HPLC. For the FIA method; the best solvent system was found to be consisting of methanol:water (10:90 v/v). A flow rate of 1.2 mL center dot min(-1) was pumped and active material was detected at 210 nm. Limit of detection (LOD) and limit of quantitation (LOQ) values were found to be 1.2x10 -7 mol center dot L-1 and 3.5x10(-7) mol center dot L-1 , respectively, for HPLC. LOD and LOQ values were found to be 2.35x10 -7 mol center dot L-1 and 7.04x10(-7) mol center dot L-1 , respectively for FIA. The results obtained from the analysis of TMZ tablet formulations were comparable using both methods.