Influence of pesticide exposure on carbonic anhydrase II from sheep stomach

Kilinc N., Isgor M. M., Sengul B., BEYDEMİR Ş.

TOXICOLOGY AND INDUSTRIAL HEALTH, vol.31, no.9, pp.823-830, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 9
  • Publication Date: 2015
  • Doi Number: 10.1177/0748233713475508
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.823-830
  • Anadolu University Affiliated: No


Carbonic anhydrase (CA) is a widely distributed enzyme and has a crucial role in the cells, tissues and organs of living organisms. It is found that CA-II is one of the most abundant CA isoenzymes in the gastrointestinal system. It plays an important role in the gastric acid secretion in stomach. In this study, we purified CA-II isoenzyme from sheep stomach with a 615.2 purification fold, 78% purification yield and 5562.02 specific activity. Moreover, the in vitro effects of some commonly used pesticides including chlorpyrifos, cypermethrin, dichlorvos, glyphosate isopropylamine and lambda cyhalomethrin on the enzyme activity were investigated. Of these compounds, glyphosate isopropylamine and dichlorvos showed an inhibition on CA-II esterase activity. They have IC50 values of 0.155 mu M and 2.690 mu M and K-i values of 0.329 mu M and 3.654 mu M, respectively. Both glyphosate isopropylamine and dichlorvos inhibited CA-II isoenzyme in a noncompetitive manner.