Imprinted polymer/organo-smectite nanocomposites for paraoxon hydrolysis

Erdem M., Say R., ERSÖZ A., DENİZLİ A., TÜRK H.

APPLIED CLAY SCIENCE, vol.47, no.3-4, pp.223-228, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 3-4
  • Publication Date: 2010
  • Doi Number: 10.1016/j.clay.2009.10.011
  • Journal Name: APPLIED CLAY SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.223-228
  • Keywords: Paraoxon, Organo-smectite, Hydrolysis, Parathion, Selectivity of imprinted polymers, EXPRESSED ORGANOPHOSPHORUS HYDROLASE, SUBSTRATE-SELECTIVE POLYMERS, NERVE AGENTS, CLAY NANOCOMPOSITES, METAL-IONS, RECOGNITION, CATALYSIS, REMOVAL, DESIGN, INTERCALATION/EXFOLIATION
  • Anadolu University Affiliated: Yes

Abstract

We have used the imprinting technique to create a polymer/organo-smectite nanocomposite catalyst. The aim was preparing a synthetic enzyme which shows high selectivity for paraoxon hydrolysis. Paraoxon imprinted polymer/organo-smectite composites (PIPO) and non-imprinted polymer/organo-smectite composites (NIPO) were prepared using methacryloylhistidine-Cu2+ (MAH-Cu(II)) and [2-methacryloyloxy)ethyl]dimethylhexadecylammonium bromide] (MOEDMAC(16)) modified smectite. These nanocomposites were applied as a catalyst in the hydrolysis of paraoxon which is an organophosphate ester and used as a pesticide. The catalytic activity of these composites was evaluated according to the enzyme kinetics model of Michaelis-Menten. PIPO showed better catalytic activity than NIPO. In addition, the paraoxon imprinted nanocomposite had greater catalytic activity (based on k(cat)/K-m values) for paraoxon hydrolysis by a factor of 391 compared to that of parathion hydrolysis. (C) 2009 Elsevier B.V. All rights reserved.