Metal chelate based site recognition of ceruloplasmin using molecularly imprinted polymer/cryogel system

DOLAK İ., CANPOLAT G., ERSÖZ A., Say R.

SEPARATION SCIENCE AND TECHNOLOGY, vol.55, no.2, pp.199-208, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 55 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.1080/01496395.2019.1577446
  • Journal Name: SEPARATION SCIENCE AND TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Agricultural & Environmental Science Database, Analytical Abstracts, Applied Science & Technology Source, Biotechnology Research Abstracts, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Pollution Abstracts, DIALNET, Civil Engineering Abstracts
  • Page Numbers: pp.199-208
  • Keywords: Ceruloplasmin, NANA-imprinted cryogel, metal-chelate complex, p-hema-mah, selective separation, SELECTIVE RECOGNITION, L-HISTIDINE, SEPARATION, CRYOGELS, REMOVAL, PURIFICATION, FERROXIDASE, GUANOSINE, POLYMERS, BINDING
  • Anadolu University Affiliated: Yes

Abstract

In the present study, N-Acetylneuraminic acid (NANA)-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidin-Cu(II)) p(HEMA-(MAH)(2)-Cu(II)) has been synthesized by radical polymerization for site recognition of ceruloplasmin. Prepared imprinted cryogel has been characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). When the binding capacity of NANA-imprinted cryogel (MIP) was compared with non-imprinted cryogel (NIP), it was found that MIP has higher adsorption capacity. The maximum amount of binding NANA has found as 83.20 mgg(-1) at pH 7.0 with flow rate of 1 mLmin(-1) at 25 degrees C. Selectivity experiments for MIP and NIP cryogel have been carried out via the NANA/D-mannose and ceruloplasmin/immunoglobulin G (IgG) pair separately and the relative selectivity coefficient (k') has found as 77.46 and 17.97, respectively. Applicable of the MIP in human serum has been studied and resulted successfully.