Akademik Veri Yönetim Sistemi
3rdInternational Congress on Multidisciplinary Natural Sciences and Engineering, Ankara, Türkiye, 7 - 08 Aralık 2023, ss.78
Along with this study, antiviral miRNAs isolations were done from the fresh above-ground parts of Euphorbia helioscopia L.(Euphorbiaceae), leaves of Salvia absconditiflora Greuter & Burdet (Syn.: S. cryptantha Monbret & Aucher ex Bentham), S. officinalis L. (Lamiaceae), Ficus benjamina L. (Moraceae) (cultured) and Olea europea L. (Oleaceae), inflorescens of Sambucus nigra L. (Adoxaceae) and Lonicera periclymenum L. (Caprifoliaceae); dry leaves of Viscum album L. (Santalaceae) (herbalist) and flowered above-ground stems of Lavandula angustifolia Mill. (Lamiaceae) (cultured) and the control group, caryopsis fruits of Triticum aestivum L. (Poaceae) (Kutluk type). The presence and expression levels of miR765, miR954, miR1086, miR1328 and miR2911 were determined by Real Time-PCR analyzes from their cDNAs. Specific stem-loop primers were designed for each miRNA and cDNA synthesis was performed using these stem-loop primers. The presence of antiviral miRNAs from the obtained cDNAs was indicated by using SYBR green-based real-time PCR. The differences of transcription/expression levels of each antiviral miRNA in subjected plants organs compared to the control group and each other were revealed. The expression profiles of different antiviral miRNAs in these plants, which grow in Turkey and have various medicinal uses, has been comparatively demonstrated for the first time. With this comparison, advantageous species of plants with high expression levels of miRNAs in case the prevention of viral infections have been present by suppressing viral genes.
* This study was supported by Anadolu University Scientific Investigation Projects Commission (Grant Number: 1809S300).