Development of a highly sensitive MIP based-QCM nanosensor for selective determination of cholic acid level in body fluids

GÜLTEKİN A., Karanfil G., SÖNMEZOĞLU S., Say R.

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, vol.42, pp.436-442, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 42
  • Publication Date: 2014
  • Doi Number: 10.1016/j.msec.2014.05.055
  • Journal Name: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.436-442
  • Keywords: Cholic acid, MIP, QCM, Nanosensor, QUARTZ-CRYSTAL MICROBALANCE, GOLD-SILVER-NANOCLUSTERS, TANDEM MASS-SPECTROMETRY, BILE-ACIDS, DOPAMINE DITHIOCARBAMATE, IMPRINTED NANOSHELL, MALDI-MS, SENSOR, CHROMATOGRAPHY, RECOGNITION
  • Anadolu University Affiliated: Yes

Abstract

Determination of cholic acid is very important and necessary in body fluids due to its both pharmaceutical and clinical significance. In this study, a quartz crystal microbalance (QCM) nanosensor, which is imprinted cholic acid, has been developed for the assignation of cholic acid. The cholic acid selective memories have been generated on QCM electrode surface by using molecularly imprinted polymer (MIP) based on methacryloylamidohistidine-copper (II) (MAH-Cu(II)) pre-organized monomer. The cholic acid imprinted nanosensor was characterized by atomic force microscopy (AFM) and then analytical performance of the cholic acid imprinted QCM nanosensor was studied. The detection limit was found to be 0.0065 mu M with linear range of 0.01-1000 mu M. Moreover, the high value of Langmuir constant (b) (7.3 * 10(5)) obtained by Langmuir graph showed that the cholic acid imprinted nanosensor had quite strong binding sites affinity. At the last step of this procedure, cholic acid levels in body fluids were determined by the prepared imprinted QCM nanosensor. (C) 2014 Elsevier B.V. All rights reserved.