© 2021, Gaurav Society of Agricultural Research Information Centre. All rights reserved.The genus Berberis (Berberidaceae) is present in Kazakhstan Flora by eight species, two of which Berberis karkaralensis Kornil. & Potapov and B. Iliensis are listed in the Red Book of Kazakhstan. But, the phytochemical studies of Kazakhstan barberry species conducted is insufficient until today. This paper presents the phytochemical analysis and antioxidant activity of fruit pulp of Berberis iliensis M. Pop and Berberis integerrima Bunge. Qualitative and quantitative chemical analysis revealed that the fruits of B. iliensis and B. integerrima Bunge contained alkanes, alkenes, alkadienes, cyclic hydrocarbons, aldehydes, fatty alcohols, triterpenoids, ketones, silicones, free monosaccharides, fatty acids and their esters. Amides and vitamin (K1) were found in some samples. The antioxidant activity of the fruit pulps was investigated by Trolox Equivalent Antioxidant Capacity (TEAC), CUPric Reducing Antioxidant Capacity (CUPRAC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) Free Radical Scavenging Activity methods. All six barberry accessions investigated showed high antioxidant activity, especially B. iliensis.