Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: An in vitro study

SÖYÜT H., Kaya E. D., BEYDEMİR Ş.

Asian Pacific Journal of Tropical Biomedicine, cilt.4, sa.8, ss.603-609, 2014 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 4 Sayı: 8
  • Basım Tarihi: 2014
  • Doi Numarası: 10.12980/apjtb.4.2014apjtb-2014-0059
  • Dergi Adı: Asian Pacific Journal of Tropical Biomedicine
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.603-609
  • Anahtar Kelimeler: Cefoperazone sodium, Inhibition, Meropenem trihydrate, Paraoxonase, Piperacillin sodium
  • Anadolu Üniversitesi Adresli: Evet

Özet

Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). Methods: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. Results: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Conclusions: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.