A Novel Phytotherapy Application: Preparation, Characterization, Antioxidant Activities and Determination of Anti-inflammatory Effects by <i>In vivo</i> HET-CAM Assay of Chitosan-based DDSs Containing Endemic <i>Helichrysum pamphylicum</i> PH Davis & Kupicha Methanolic Extract

Ismailovi N., KIYAN H. T., ÖZTÜRK A. A.

CURRENT DRUG DELIVERY, vol.21, no.6, pp.901-916, 2024 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 21 Issue: 6
  • Publication Date: 2024
  • Doi Number: 10.2174/1567201820666230328122504
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE
  • Page Numbers: pp.901-916
  • Keywords: Antioxidant, chitosan gel, F9-HP, Helichrysum pamphylicum P.H. Davis & Kupicha, HET-CAM, polysaccharide
  • Anadolu University Affiliated: Yes


Background Numerous pharmaceutical applications for chitosan, a polysaccharide made from the shells of crustaceans by deacetylating chitin that occurs naturally, are currently being researched. Chitosan, a natural polymer, is successfully used to prepare many drug-carrier systems, such as gel, film, nanoparticle, and wound dressing. Objective Preparing chitosan gels without external crosslinkers is less toxic and environmentally friendly. Methods Chitosan-based gels containing Helichrysum pamphylicum P.H. Davis & Kupicha methanolic extract (HP) were produced successfully. Results The F9-HP coded gel prepared with high molecular weight chitosan was chosen as the optimum formulation in terms of pH and rheological properties. The amount of HP was found to be 98.83% +/- 0.19 in the F9-HP coded formulation. The HP release from the F9-HP coded formula was determined to be slower and 9 hours prolonged release compared to pure HP. It was determined that HP release from F9-HP coded formulation with the DDSolver program was by anomalous (non-fickian) diffusion mechanism. The F9-HP coded formulation significantly showed DPPH free radical scavenger, ABTS center dot+ cation decolorizing and metal chelating antioxidant activity while weakly reducing antioxidant potential. According to the HET-CAM scores, strong anti-inflammatory activity was obtained by the F9-HP coded gel at a dose of 20 mu g.embryo(-1) (p<0.05 compared with SDS). Conclusion In conclusion, it can be said that chitosan-based gels containing HP, which can be used in both antioxidant and anti-inflammatory treatment, were successfully formulated and characterized.