Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles

YAZICIOĞLU F. G., Ozgur E., Yilmaz E., UZUN L., Say R., Denizli A.

BIOSENSORS & BIOELECTRONICS, vol.26, no.2, pp.815-821, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1016/j.bios.2010.06.003
  • Journal Name: BIOSENSORS & BIOELECTRONICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.815-821
  • Keywords: QCM, Nanoparticles, Lysozyme, Molecular imprinting, Nanosensors, SURFACE-PLASMON RESONANCE, CADMIUM REMOVAL, QCM SENSOR, RECOGNITION, PROTEINS, SERUM, IMMUNOSENSOR, DIAGNOSIS, POLYMERS, ALBUMIN
  • Anadolu University Affiliated: Yes

Abstract

The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37 degrees C for 6 h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 mu g/mL) and natural sources (egg white) (460-1500 ng/mL). (C) 2010 Elsevier B.V. All rights reserved.