A study of calcium release from rat liver microsomes by thapsigargicin induction

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Oztetik E.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.59, sa.2, ss.135-140, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 59 Sayı: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1501/vetfak_0000002515
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.135-140
  • Anahtar Kelimeler: Ca2+ release, intracellular stores, SERCA ATPases, spectroscopy, thapsigargin (TG), thapsigargicin (TGC), TUMOR PROMOTER, CYCLOPIAZONIC ACID, L1210 CELLS, INHIBITION, ATPASE, CA-2+, 2,5-DI(TERT-BUTYL)-1,4-BENZOHYDROQUINONE, GENERATION, TRANSPORT
  • Anadolu Üniversitesi Adresli: Evet

Özet

The primary aim of the study was to test the influences of thapsigargicin in releasing Ca2+ from the intracellular Ca2+ stores and comparing it with the effects of thapsigargin. In this study, the effect of tumour promoter thapsigargicin on intracellular Ca2+ has been described. Therefore, rat liver endoplasmic reticulum subcellular fractions (microsomes) were employed and Ca2+ movements measured by spectrofluorimeter. Fluo-3 studies were used to follow Ca2+ release in experiments with microsomes. When results are evaluated, it has been concluded that like thapsigargin, thapsigargicin has the ability of discharging the intracellular Ca2+ stores, increasing the intracellular free Ca2+ concentration ([Ca2+]i) and being a potent and specific inhibitor of the ER Ca2+-ATPase.