A study of calcium release from rat liver microsomes by thapsigargicin induction


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Oztetik E.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.59, no.2, pp.135-140, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 59 Issue: 2
  • Publication Date: 2012
  • Doi Number: 10.1501/vetfak_0000002515
  • Journal Name: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.135-140
  • Keywords: Ca2+ release, intracellular stores, SERCA ATPases, spectroscopy, thapsigargin (TG), thapsigargicin (TGC), TUMOR PROMOTER, CYCLOPIAZONIC ACID, L1210 CELLS, INHIBITION, ATPASE, CA-2+, 2,5-DI(TERT-BUTYL)-1,4-BENZOHYDROQUINONE, GENERATION, TRANSPORT
  • Anadolu University Affiliated: Yes

Abstract

The primary aim of the study was to test the influences of thapsigargicin in releasing Ca2+ from the intracellular Ca2+ stores and comparing it with the effects of thapsigargin. In this study, the effect of tumour promoter thapsigargicin on intracellular Ca2+ has been described. Therefore, rat liver endoplasmic reticulum subcellular fractions (microsomes) were employed and Ca2+ movements measured by spectrofluorimeter. Fluo-3 studies were used to follow Ca2+ release in experiments with microsomes. When results are evaluated, it has been concluded that like thapsigargin, thapsigargicin has the ability of discharging the intracellular Ca2+ stores, increasing the intracellular free Ca2+ concentration ([Ca2+]i) and being a potent and specific inhibitor of the ER Ca2+-ATPase.