In Vitro Biological Effects of Endemic Anatolian Spotted Newt Dermal Venom: A Potential Active Pharmaceutical Ingredient (API) for Drug Delıvery Systems

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Karış M., Çimik A., Gürel Gürevin E., Öztürk A. A., Kıyan H. T.

13th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Turkey, 22 - 25 June 2021, no.129, pp.114-115

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.114-115
  • Anadolu University Affiliated: Yes


Introduction: Neurergus strauchii strauchii Steindachner, which is a member of the Salamandrid genus, has a haemolytic dermal venom (1). Previously, the dermal venom microflora of Neurergus sp. has been reported to exhibit antimicrobial activities against pathogene microorganisms (2) The main goal of this study is research on the in vitro anti-inflammatory, antioxidant and antimicrobial activities of dermal venom of Neurergus strauchii.

Materials and Methods: In vitro anti-inflammatory, antioxidant and antibacterial effects of dermal venom of Neurergus strauchii were determined by using in vitro COX-1 & COX-2, DPPH scavenging assays and agar microdilution method. SC-560, celecoxib, Vit C and chloramphenicol were used as positive controls. Bifidobacterium animalis ssp lactis B94 was used as obligatory as well as Lactobacillus reuterii DSM 17938 was used as a facultative anaerobic microorganism.

Results: The total protein amount of the secretion was found at 1700 μg/mL. IC50 results of venom were calculated as 157.9770, 96.84, 11.6873 μg/mL for in vitro COX-1 & COX-2, DPPH inhibitons respectively. Antimicrobial activity MIC results of venom were determined as >400μg/mL against both of the microorganisms.

Conclusions: Venom inhibited all enzyms and DPPH on related concentrations. It is safe on obligatory and facultative anaerobic microorganisms. This result supports the texistence of probiotic dermal venom microflora (2). The results of this study indicated that link between related enzyme activities and probiotic microorganism protective activity. More detailed analysis will be cconducted in the future stages of the study, and different drug delivery systems will be designed and characterized by using the relevant substance as an API.