Determination of Folic Acid by CE in Various Cultivated Variety of Lentils

UYSAL Ü. D., Oncu-Kaya E. M., TUNÇEL M.

CHROMATOGRAPHIA, vol.71, no.7-8, pp.653-658, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 71 Issue: 7-8
  • Publication Date: 2010
  • Doi Number: 10.1365/s10337-010-1555-4
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.653-658
  • Keywords: Capillary electrophoresis, Folic acid, Lentils, WATER-SOLUBLE VITAMINS, CAPILLARY-ELECTROPHORESIS, FOLATE, HPLC, FOOD, FORTIFICATION, EXTRACTION, STABILITY, NOODLES, TABLETS
  • Anadolu University Affiliated: Yes

Abstract

A simple extraction and determination method for folic acid (FA) in lentil samples was developed employing capillary electrophoresis. The analysis was performed in a 75 mu m ID fused silica capillary using a running buffer of 10 mM sodium borate (10%, v/v, methanol, pH 9) at +18 kV and a detection wavelength at 200 nm. Methylparaben was used as the internal standard. FA signal response was linear in the range between 1.2 x 10(-5) and 4.8 x 10(-5) M. Limit of detection (inter-day) was determined at 6.12 x 10(-7) M (3.3 sigma s(-1)). The amount of FA found in green, red and mignon lentils was found to range between 0.408 and 0.742 mg g(-1).