Determination of Folic Acid by CE in Various Cultivated Variety of Lentils
CHROMATOGRAPHIA, cilt.71, sa.7-8, ss.653-658, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 71 Sayı: 7-8
- Basım Tarihi: 2010
- Doi Numarası: 10.1365/s10337-010-1555-4
- Dergi Adı: CHROMATOGRAPHIA
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.653-658
- Anahtar Kelimeler: Capillary electrophoresis, Folic acid, Lentils, WATER-SOLUBLE VITAMINS, CAPILLARY-ELECTROPHORESIS, FOLATE, HPLC, FOOD, FORTIFICATION, EXTRACTION, STABILITY, NOODLES, TABLETS
- Anadolu Üniversitesi Adresli: Evet
Özet
A simple extraction and determination method for folic acid (FA) in lentil samples was developed employing capillary electrophoresis. The analysis was performed in a 75 mu m ID fused silica capillary using a running buffer of 10 mM sodium borate (10%, v/v, methanol, pH 9) at +18 kV and a detection wavelength at 200 nm. Methylparaben was used as the internal standard. FA signal response was linear in the range between 1.2 x 10(-5) and 4.8 x 10(-5) M. Limit of detection (inter-day) was determined at 6.12 x 10(-7) M (3.3 sigma s(-1)). The amount of FA found in green, red and mignon lentils was found to range between 0.408 and 0.742 mg g(-1).