A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples

LEVENT S., ÖZCAN S., Geven A., CAN N. Ö.

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, vol.24, no.3, pp.386-399, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 24 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.2174/1386207323999201110192558
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.386-399
  • Keywords: Ceftiofur, solvent-based calibration, LC-MS/ MS, matrix-matched calibration, milk analysis, monolithic silica column, BETA-LACTAM ANTIBIOTICS, MULTIRESIDUE ANALYSIS
  • Anadolu University Affiliated: Yes


Introduction: In the present study, a sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was described for the determination of ceftiofur (CEF) in cow milk and pharmaceutical preparations. CEF is an antibiotic compound, which is commonly used in the treatment of animal diseases such as respiratory system, soft tissue, and foot infections, as well as postpartum acute puerperal metritis. One of the critical features of CEF is its prescription while breastfeeding cows; in accordance, its quantitative estimation is essential to assess its residual amounts.