Rapid and high quality DNA isolation from Origanum onites for RAPD and ISSR analysis

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SÖZEN E., Poyraz I.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, cilt.63, sa.7-8, ss.595-598, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 7-8
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1515/znc-2008-7-821
  • Dergi Adı: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.595-598
  • Anahtar Kelimeler: CTAB DNA extraction, Origanum onites, RAPD, ISSR, ESSENTIAL OILS, GENOMIC DNA, PLANTS, POLYSACCHARIDE, PROTOCOL
  • Anadolu Üniversitesi Adresli: Evet

Özet

Origanum unites is an economically important medicinal plant with high essential oil content. Lack of an appropriate DNA isolation procedure is a limiting factor for any molecular study of this plant. We have used a protocol for genomic DNA isolation based on a hexade-cyltrimethylammonium bromide (CTAB) method described for other plant species. The method involves mortar grinding of leaf tissue, modified CTAB extraction using high salt concentrations and polyvinyl pyrrolidone, and successive isoamyl alcohol/chloroform extractions. The yield was approx. 20 mu g DNA per 200 mg of initial fresh plant material. The genomic DNA obtained by this method was suitable to be used in restriction digests, inter simple sequence repeat (ISSR) and randomly amplified polymorphic DNA (RAPD) reactions. This extraction method should facilitate the molecular analysis of Origanum chemotypes.