© 2017, University of Istanbul. All rights reserved.In this study, we have synthesized seven novel 2-[(5-(4-chlorobenzyl)-1,3,4-oxadiazol-2-yl)thio]-N-(6-substituted benzothiazol-2-yl)acetamide derivatives (4ag) starting from ethyl 4-chlorophenyl acetate. The antimicrobial acitivity of the compounds was screened against seven Gram positive and Gram negative bacteria and four fungi species; Escherichia coli ATCC 25922, Escherichia coli ATCC 35218, Enterococcus faecalis ATCC 51299, Enterococcus faecalis ATCC 29212, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Candida albicans, Candida krusei, Candida glabrata, Candida parapsilosis. Minumum inhibitor concentration (MIC) was calculated and compared with standard drugs, chloramphenicol and ketoconazole. Regarding the results of MIC, all compounds exhibited potency either at the higher concentrations or at the same concentrations compared with positive controls.