In Vivo Anti-Angiogenic and Anti-Inflammatory Potentials of R(+) or S(-) Limonene Loaded EUDRAGIT® RS 100 Nanoparticles

Creative Commons License

Kıyan H. T., Öztürk A. A.

13th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Turkey, 22 - 25 June 2021, pp.122-123

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.122-123
  • Anadolu University Affiliated: Yes


Introduction: R(+) and S(-) limonene are major compounds of M. spicata essential oil have been reported to be used in treatment due to their antimicrobial, anticancer, anti-angiogenic, anti- inflammatory and antioxidant effects and as well as in cosmetics and as sweetener in drinks (1). We aimed to design and characterize R(+) or S(-) limonene loaded nanoparticles (NPs) and investigate their in vivo anti-angiogenic and anti- inflammatory potentials.

Materials and Methods: Eudragit RS 100-based NPs were prepared by following the nanoprecipitation technique with some modifications (2). The in vivo anti-angiogenic and anti-inflammatory activities of R(+) and S(-) limonene NPs were evaluated by using in vivo CAM and HET-CAM assays respectively (3,4).

Results: According to the results, NPs at the concentration of 50 μg/pellet including R(+) limonene and S(-) limonene as 4 μg/pellet showed strong in vivo anti-angiogenic potentials with anti- angiogenic scores of 0.93 ± 0.1 and 0.74 ± 0.05 and no membrane irritation and embryotoxicity compared with (±)-Thalidomide (0.8 ± 0.05) (Figure 1). R(+) and S(-) limonene NPs also exhibited strong in vivo anti-inflammatory activities with 75.00 ± 12.50% and 80.00 ± 11.18% inhibition values compared with 81.25 ± 8.84%.

Conclusions: Plant derived natural angiogenesis inhibitors including plant extracts, essential oils and their volatile compounds used to treat cancer and inflammatory diseases, are seen promising as good alternatives to synthetic ones due to their low side effect profiles and different mechanisms of effect.

Acknowledgements: This study was financed by Anadolu University Scientific Research Project Foundation (No: 1905S054).