Construction of a metagenomic library for the marine sponge Halichondria okadai

Abe T., Sahin F. P., Akiyama K., Naito T., Kishigami M., Miyamoto K., ...Daha Fazla

Bioscience, Biotechnology and Biochemistry, cilt.76, sa.4, ss.633-639, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 76 Sayı: 4
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1271/bbb.110533
  • Dergi Adı: Bioscience, Biotechnology and Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.633-639
  • Anahtar Kelimeler: 454-pyrosequencing, Fosmid, Halichondria okadai, Metagenomic library, Sponge
  • Anadolu Üniversitesi Adresli: Hayır

Özet

Symbionts of the marine sponge Halichondria okadai are promising as a source of natural products. Metagenomic technology is a powerful tool for accessing the genetic and biochemical potential of bacteria. Hence, we established a method of recovering bacterial-enriched metagenomic DNA by stepwise centrifugation. The metagenomic DNA was analyzed by ultrafast 454- pyrosequencing technology, and the results suggested that more than three types of bacterial DNA, Alphaproteobacteria, Actinobacteria, and Cyanobacteria, had been recovered, and that eukaryotic genes comprised only 0.02% of the metagenomic DNA. These results indicate that stepwise centrifugation and real-time quantitative PCR were effective for separating sponge cells and symbiotic bacteria, and that we constructed a bacteria-enriched metagenomic library from a marine sponge, H. okadai, selectively for the first time.