Akademik Veri Yönetim Sistemi
International Journal of Scientific Research in Chemistry, cilt.7, sa.2, ss.1-14, 2022 (Hakemli Dergi)
This study was undertaken for screening biological activity of Azadirachta indica A. Juss. growing in Somalia. The study was aimed to investigate the biological activity namely: antioxidant activity, total phenol content, total flavonoid content and as well as its Phytochemical composition, 43mg of essential oils (EOs) were extracted from 65g of air-dried plant by hydrodistillation for 3hrs and analyzed with gas chromatography-mass spectrometry (GC-MS), gas chromatography- flame ionization detector (GC-FID), and the total of 31components represented 90.6 % of the total oil were identified. The main components were -Elemene (32.6 %), germacrene-B (30,1%), whereas the MeOH and Water extracts were employed with High-Pressure Liquid Chromatography (HPLC) to determine phenolic compounds of the plants. The phenolic substances were identified and quantified after comparison with reference standards. Th radical scavenging effect of the extract was analyzed spectrophotometrically with DPPH and TEAC assay respectively. The scavenging effect was fully active in TEAC assay and in all DPPH assays except for the essential oil extracts. Total phenolic content in the extracts was determined by spectrometric method applying the Folin- Ciocalteu assay. The result was ranged from 0.108 to 0.139mg gallic acid/mL and was slightly greater than that in total flavonoid, the Flavonoid contents of the plant was also determined spectrophotometrically using rutin as a standard reference compound with aluminum chloride colorimetric assay. It ranged from 0.003 to 0.052 mg/ml. This study is of significance in providing information on the Azadirachta Indica A. Juss native to Somalia and it appears to be a rich source of antioxidants and phytochemical studies may supplement the remedial needs of the human.