Retention Behaviour of Bupropion Hydrochloride in Reversed Phase Ion Pair LC and Validated Analysis of the Drug in Pharmaceuticals

Yeniceli D., Dogrukol-Ak D.

CHROMATOGRAPHIA, sa.1-2, ss.79-84, 2010 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1365/s10337-009-1410-7
  • Dergi Adı: CHROMATOGRAPHIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.79-84
  • Anahtar Kelimeler: Column liquid chromatography, Ion-pairing, Pharmaceutical analysis, Method validation, Bupropion hydrochloride, HUMAN-PLASMA, LIQUID-CHROMATOGRAPHY, MAJOR METABOLITES, SEPARATION, COLUMN, HPLC, PHARMACOKINETICS, OPTIMIZATION
  • Anadolu Üniversitesi Adresli: Evet

Özet

The chromatographic behaviour of bupropion hydrochloride, a basic drug of pK (a) 7.9, has been investigated under reversed-phase ion-pairing conditions and the results were used to develop a method for analysis of bupropion hydrochloride in pharmaceuticals. Chromatographic separation of bupropion hydrochloride and carbamazepine (used as internal standard) was performed on a C-8 column (150 mm x 4.6 mm i.d., 3.5-mu m particle), with 40:10:50 (v/v) methanol-acetonitrile-phosphate buffer (20 mm, pH 3.0), containing 10 mm 1-heptane sulfonic acid sodium salt (1-HSA), as optimum mobile phase at a flow rate of 1.0 mL min(-1). UV detection was at 254 nm. The fully validated method enables reproducible and selective analysis of bupropion hydrochloride in pharmaceuticals.