Molecularly imprinted ligand-exchange recognition assay of glucose by quartz crystal microbalance


Ersoz A., Denizli A., Ozcan A., Say R.

BIOSENSORS & BIOELECTRONICS, cilt.20, sa.11, ss.2197-2202, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 11
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1016/j.bios.2004.07.030
  • Dergi Adı: BIOSENSORS & BIOELECTRONICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2197-2202
  • Anahtar Kelimeler: molecular imprinting, MIP, QCM, glucose sensor, ligand exchange, SEPARATION, POLYMERS, SITES, SENSORS, BEADS
  • Anadolu Üniversitesi Adresli: Hayır

Özet

Molecular imprinted polymers (MIP) as a recognition element for sensors are increasingly of interest and MIP-quartz crystal microbalance (QCM) have started to appear in the literature. In this study, we have combined quartz crystal microbalance with MIP to prepare a sensor using the ability of glucose to chelate of copper (II) ion of methacrylamidohistidine (MAH) monomer to create ligand exchange (LE) assembled monolayer which is suitable for glucose determination. The study includes the measurement of binding interaction of molecularly imprinted QCM sensor via ligand interaction, investigation of the pH effect on frequency shift and recognition selectivity studies of glucose-imprinted polymer with respect to methyl-alpha-(D)-glueopyranoside and sucrose. B-max (number of binding sites) and K-D (dissociation constant of the metal-chelate copolymer) were also calculated using Scathard plot and the detection limit was found as 0.07 mM. MIP showed higher glucose-binding affinity than a well-known glucose binding protein, conconavalin A. (c) 2004 Elsevier B.V. All rights reserved.