Determination of piribedil in human serum, urine and pharmaceutical dosage form by LC-DAD

Altiokka G., CAN N. Ö., Aboul-Enein H. Y.

CHROMATOGRAPHIA, vol.67, no.11-12, pp.905-910, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 67 Issue: 11-12
  • Publication Date: 2008
  • Doi Number: 10.1365/s10337-008-0626-2
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.905-910
  • Keywords: column liquid chromatography, pharmaceutical analysis, biological fluids, piribedil, PERFORMANCE LIQUID-CHROMATOGRAPHY, PIPERAZINE DERIVATIVES, METABOLITES, PLASMA, DRUGS
  • Anadolu University Affiliated: Yes

Abstract

A rapid, selective and sensitive reversed-phase liquid chromatographic (LC) method was developed for the determination of piribedil in human serum, urine and pharmaceutical dosage form. LC analysis was carried out using reversed-phase isocratic elution with a C-18 column and a mobile phase of 0.01 M phosphate buffer-acetonitrile (50:50, v/v). The chromatograms showed good resolution and sensitivity with no interference of human serum and urine. Piribedil concentrations were determined using diode array detection at 240 nm. Sildenafil citrate was used as internal standard. The limit of quantification (LOQ) and limit of detection (LOD) concentrations were 107.2 and 321.6 pg mL(-1), 96.6 and 290.4 pg mL(-1), 161.7 and 53.9 pg mL(-1) for urine, serum and pharmaceutical dosage forms, respectively. The method was validated for its linearity, precision and accuracy and applied to the tablets, urine and human serum. In addition, the results were compared to those obtained from UV-spectrophotometry.