This study was undertaken to determine the flavonoids in the species of genus Alchemilla L. section Alchemilla L. subsection Calycanthum Rothm. Ser. Elatae Rothm., using the identification techniques such as high performance liquid chromatography (HPLC) and thin layer chromatography (TLC). Eight endemic Turkish species were studied for their flavonoid profiles. They are A. armeniaca, A. erzincanensis, A. cimilensis, A. orduensis, A. ikizdereensis, A. oriturcica, A. bursensis and A. hirsutiflora. It is the first time that flavonoid profiles of these species have been identified. Flavonol O-glycosides and flavone C- glycosides were identified in Alchemilla endemic species. The flavonoid components determined in the species are rutin (quercetin-3-O-rutinoside), hyperoside (quercetin-3-O-galactoside), isoquercetin (quercetin-3-O-glucopyranoside), quercitrin (quercetin-3-O-rhamnoside) as flavonol O-glycosides, and orientin (luteolin-8-C-glucoside), vitexin (apigenin-8-C-glucoside) as the flavone C-glycosides.