Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis


Incesu Z., Hatipoglu I., SİVAS H., ERGENE E., AKALIN ÇİFTÇİ G.

INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY, no.10, pp.789-796, 2013 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2013
  • Journal Name: INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.789-796
  • Keywords: Apoptosis, Cell culture, Fibronectin, Osteosarcoma, Type IV collagen, NEOADJUVANT CHEMOTHERAPY, OSTEOBLASTIC CELLS, MECHANISMS, EXPERIENCE, SURVIVAL, ADHESION, EXTREMITIES, ACTIVATION, EXPRESSION, INTEGRINS
  • Anadolu University Affiliated: Yes

Abstract

The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.