Caffeoylquinic Acids, Cytotoxic, Antioxidant, Acetylcholinesterase and Tyrosinase Enzyme Inhibitory Activities of Six Inula Species from Bulgaria

Trendafilova A., Ivanova V., Rangelov M., Todorova M., ÖZEK G., Yur S., ...Daha Fazla

CHEMISTRY & BIODIVERSITY, cilt.17, sa.4, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 4
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/cbdv.202000051
  • Dergi Adı: CHEMISTRY & BIODIVERSITY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Inula species, caffeoylquinic acids, antioxidant activity, acetylcholinesterase and tyrosinase inhibition, cytotoxicity, OCULUS-CHRISTI, EXTRACT, L., BRITANNICA, PROFILE, MS/MS
  • Anadolu Üniversitesi Adresli: Evet

Özet

Chlorogenic (5-CQA), 1,5-, 3,5-, 4,5- and 3,4-dicaffeoylquinic (DCQA) acids were identified and quantified in the methanol extracts of Inula oculus-christi L., I. bifrons L., I. aschersoniana Janka var. aschersoniana, I. ensifolia L., I. conyza (Griess.) DC. and I. germanica L. by HPLC analysis. The amount of 5-CQA varied from 5.48 to 28.44 mg/g DE and the highest content was detected in I. ensifolia. 1,5-DCQA (4.05-55.25 mg/g DE) was the most abundant dicaffeoyl ester of quinic acid followed by 3,5-DCQA, 4,5-DCQA and 3,4-DCQA. The extract of I. ensifolia showed the highest total phenolic content (119.92 +/- 0.95 mg GAE/g DE) and exhibited the strongest DPPH radical scavenging activity (69.41 +/- 0.55 %). I. bifrons extract was found to be the most active sample against ABTS(.+) (TEAC 0.257 +/- 0.012 mg/mL) and the best tyrosinase inhibitor. The studied extracts demonstrated a low inhibitory effect towards acetylcholinesterase and possessed low cytotoxicity in concentration range from 10 to 300 mu g/mL toward non-cancer (MDCK II) and cancer (A 549) cells.