Akademik Veri Yönetim Sistemi
54. International Symposium on Essential Oils (ISEO2024), Veszprém, Macaristan, 8 - 11 Eylül 2024, sa.24, ss.154
Objective
Wound healing is a major issue in skin injuries as it is well-known. Natural products have been utilized since early
times of humanity. Chitosan is a biopolymer, which is used internally and externally for its antimicrobial as well
its anti-inflammatory properties along with is biofilm forming features. Hamamelis virginiana L., native to N.
America, is known to have medicinally valuable products, which contains various bioactive compounds such as
volatiles. The aim of the study is to optimize the chitosan – Hamamelis combination according to its bioactivity.
Methods
The commercially available chitosan, Hamamelis distillate and pluronic constituents were formulated for potential
external use. In vitro antimicrobial evaluation against human skin pathogens were performed initially using a
microdilution assay, where ingredients and formulations were evaluated using a checkerboard assay. In addition,
in vitro lipoxygenase enzyme inhibition experiments were performed for the anti-inflammatory potential and a cell
line MTT assay for toxicity evaluation.
Results
According to the first formulation results the human pathogens Escherichia coli NRRL B-3008, Staphylococcus
aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Corynebacterium striatum ATCC BAA 1293,
Candida albicans ATCC 90028 were susceptible with a MIC >1000 mg/mL concentration. Various combinations
towards bioactive formulations are ongoing, also for anti-inflammatory, and toxicity evaluations. To the best of
our knowledge, the combination of chitosan with Hamamelis preparations is for the first time.
Conclusions
This type formulations have potential acute and chronic application in various skin conditions, however, more
detailed bioactivity tests are needed.
ACKNOWLEDGEMENTS
This study was supported by Anadolu Univ. Scientific Research Projects Commission no: KGT-2023-5