Wound care Formulation using Chitosan and Hamamelis sp. Preprations


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Turhal C., Büyükköroğlu G., Demirci F.

54. International Symposium on Essential Oils (ISEO2024), Veszprém, Hungary, 8 - 11 September 2024, no.24, pp.154

  • Publication Type: Conference Paper / Summary Text
  • City: Veszprém
  • Country: Hungary
  • Page Numbers: pp.154
  • Anadolu University Affiliated: Yes

Abstract

Objective

Wound healing is a major issue in skin injuries as it is well-known. Natural products have been utilized since early

times of humanity. Chitosan is a biopolymer, which is used internally and externally for its antimicrobial as well

its anti-inflammatory properties along with is biofilm forming features. Hamamelis virginiana L., native to N.

America, is known to have medicinally valuable products, which contains various bioactive compounds such as

volatiles. The aim of the study is to optimize the chitosan – Hamamelis combination according to its bioactivity.

Methods

The commercially available chitosan, Hamamelis distillate and pluronic constituents were formulated for potential

external use. In vitro antimicrobial evaluation against human skin pathogens were performed initially using a

microdilution assay, where ingredients and formulations were evaluated using a checkerboard assay. In addition,

in vitro lipoxygenase enzyme inhibition experiments were performed for the anti-inflammatory potential and a cell

line MTT assay for toxicity evaluation.

Results

According to the first formulation results the human pathogens Escherichia coli NRRL B-3008, Staphylococcus

aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Corynebacterium striatum ATCC BAA 1293,

Candida albicans ATCC 90028 were susceptible with a MIC >1000 mg/mL concentration. Various combinations

towards bioactive formulations are ongoing, also for anti-inflammatory, and toxicity evaluations. To the best of

our knowledge, the combination of chitosan with Hamamelis preparations is for the first time.

Conclusions

This type formulations have potential acute and chronic application in various skin conditions, however, more

detailed bioactivity tests are needed.

ACKNOWLEDGEMENTS

This study was supported by Anadolu Univ. Scientific Research Projects Commission no: KGT-2023-5