DETERMINATION OF ZEARALENONE BY THE CAPILLARY ZONE ELECTROPHORESIS-UV DETECTION AND ITS APPLICATION TO POULTRY FEED AND CEREALS

GÜRAY T., TUNÇEL M., UYSAL Ü. D., ÖNCÜ KAYA E. M.

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, cilt.36, sa.10, ss.1366-1378, 2013 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 10
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1080/10826076.2012.691437
  • Dergi Adı: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1366-1378
  • Anahtar Kelimeler: capillary electrophoresis, grain, maize flour, mycotoxin, poultry feed, zearalenone, PERFORMANCE LIQUID-CHROMATOGRAPHY, MACROCYCLIC LACTONE MYCOTOXINS, TANDEM MASS-SPECTROMETRY, GAS-CHROMATOGRAPHY, FUSARIUM TOXINS, ANIMAL FEED, BABY FOOD, OCHRATOXIN, CORN, AFLATOXINS
  • Anadolu Üniversitesi Adresli: Evet

Özet

A capillary zone electrophoretic method for the determination of zearalenone is described in this study. In the separation, a run buffer consisting of 20mM sodium tetraborate at pH 9.0 with 15% acetonitrile applying 15kV, injecting 10s at 50 mbar was utilized. Phenobarbital was a suitable internal standard and the signals were recorded at 254nm. Zearalanon and internal standard appeared at 5.46min (RSD% 0.20) and 6.35min (RSD% 0.18), respectively. The repeatability results are in the range of 0.011.58 for inter-day. The linearity was investigated in the range of 5.20x107M to 7.86x105M ZEA and it was linear fitting to the equation of [rPN=244803.8 C(M) 0.0348; r=0.9999]. The LOD and LOQ were calculated to be 2.70x108M (8.25 mu g/L) and 8.17x108M (25 mu g/L), respectively. Then, the method was successfully applied to the analysis of ZEA in poultry feeds, flour of maize, grain, and certain cereal samples such as fibrous biscuit, popcorn, and rice crisp.