Ion-imprinted supermacroporous cryogel, for in vitro removal of iron out of human plasma with beta thalassemia

ASLIYÜCE ÇOBAN S., BERELİ N., UZUN L., ONUR M. A., Say R., DENİZLİ A.

SEPARATION AND PURIFICATION TECHNOLOGY, vol.73, no.2, pp.243-249, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 73 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1016/j.seppur.2010.04.007
  • Journal Name: SEPARATION AND PURIFICATION TECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.243-249
  • Keywords: Molecular imprinting, Molecular recognition, Iron removal, Metal detoxification, Beta thalassemia, SOLID-PHASE EXTRACTION, CADMIUM REMOVAL, AFFINITY, CHROMATOGRAPHY, RECOGNITION, BEADS, BILIRUBIN, POLYMERS, MONOLITH, PRECONCENTRATION
  • Anadolu University Affiliated: Yes

Abstract

The aim of this study is to prepare an ion-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-cysteine methyl ester) [PHEMAC] cryogel which can be used to remove Fe3+ ions from beta thalassemia patient plasma in vitro. Fe3+, as a template, was initially complexed with N-methacryloyl-(L)-cysteine methyl ester (MAC) to form the complex monomer MAC-Fe3+ polymerized to constitute a Fe3+ ion-imprinted PHEMAC-Fe3+ cryogel by cryo-polymerization. The template (Fe3+) was removed from the polymer by using 0.1 M EDTA solution. The specific surface area of PHEMAC-Fe3+ cryogel was found to be 32.7 m(2)/g. A maximum Fe3+ adsorption capacity of 75 mu g/g was observed with PHEMAC-Fe3+ cryogel. It was determined that PHEMAC-Fe3+ cryogel possesses high selectivity coefficients for Fe3+/Cd2+ and Fe3+/Ni2+, which are 10.5 and 2.3, respectively, times greater than PHEMAC (non-imprinted cryogel produced in the absence of Fe3+). (C) 2010 Elsevier B.V. All rights reserved.