Investigation of the Apoptotic Effect of Curcumin in Human Leukemia HL-60 Cells by Using Flow Cytometry

DİKMEN M., CANTÜRK Z., ÖZTÜRK Y., TUNALI Y.

CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS, cilt.25, sa.6, ss.749-755, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 6
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1089/cbr.2010.0822
  • Dergi Adı: CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.749-755
  • Anahtar Kelimeler: curcumin, HL-60, apoptosis, caspase-3, MTT, flow cytometry, ENDOPLASMIC-RETICULUM STRESS, OVARIAN-CANCER CELLS, CYTOCHROME-C, IN-VITRO, LINES, DEATH, PROLIFERATION, ACTIVATION, GENERATION, INDUCTION
  • Anadolu Üniversitesi Adresli: Evet

Özet

Curcumin (diferuloylmethane), the major yellow pigment isolated from the turmeric (Curcuma longa), has received much attention due to several biological properties. Curcumin exhibits a variety of pharmacological effects including antitumor, anti-inflammatory, and anti-infectious activities. In the present study, the effects of curcumin on apoptosis in the acute promyelocytic human leukemia (HL-60) cells was evaluated. Cytotoxic effects of curcumin on HL-60 cells were determined by MTT. HL-60 cells underwent apoptosis on treatment with curcumin, as indicated by increased annexin V-binding capacity and caspase-3 activation with flow cytometric analysis. Concentrations of 15, 20, and 40 mu M curcumin significantly reduced cell proliferations. When HL-60 cells were treated with 10, 15, 20, and 40 mu M concentration of curcumin, apoptotic rates were determined as 1.2, 81.1, 84.5, and 88.6%, respectively. On the incubations with the concentrations of curcumin, caspase-3 expressions (+) were found to be elevated by 8.5, 18.6, 91.2, and 92.4%, respectively. It was shown that curcumin had significant cytotoxic and apoptotic effects on HL-60 cells. It was suggested that curcumin may have a potential therapeutic role for human leukemia.